WebProtinfo PsiCSI - Assign secondary structure using NMR chemical shift data and neural networks Protinfo PsiCSI Hung L-H, Samudrala R. PROTINFO: Secondary and tertiary protein structure prediction. Nucleic Acids Research 31: 3296-3299, 2003. Hung L-H, Samudrala R. Accurate and automated assignment of secondary structure with PsiCSI. WebPsiCSI is a highly accurate and automated method of assigning secondary structure from NMR data, which is a useful intermediate step in the determination of tertiary structures. The method combines information from chemical shifts and protein sequence using three layers of neural networks.

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WebPsiCSI begins by refining the CSI methodology. Rather than three indices, three sepa-rate potentials ranging from 0 to 1 are assigned to reflect the relative likelihood of a given chemical-shift value being associated with a given secondary structure state. Like CSI, PsiCSI reduces noise by polling nearby shifts. PsiCSI ex- WebCSI 3.0 is an accurate, automated, easy-to-use web ser- vice for calculating structural information from chemical shiftdata.Inparticular,CSI3.0accuratelydetermineseight... rave party wear

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