Hif 1a antibody

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August undefined, 2024

WebTo determine whether HIF-1α binds these HREs, we performed a CHIP assay using anti-HIF-1α antibodies in A549 cells cultured under hypoxic (1.5% O 2) or normoxic (21% O 2) conditions. DNA fragments containing the HRE1 and/or HRE2 binding sites were significantly more abundant under hypoxic conditions ( Figure 4G ), suggesting that HIF … WebHIF-1α Antibody (28b) is an IgG 1 κ mouse monoclonal HIF-1 alpha antibody (also designated HIF1A antibody or HIF-1 alpha antibody) suitable for the detection of the …

Anti-HIF1A Antibody (A80910) Antibodies.com

WebHIF-1 is a heterodimer consisting of an alpha and beta subunit, both belonging to the basic-helix-loop-helix Per-aryl hydrocarbon receptor nuclear translocator-Sim (PAS) family of … WebHydroxylates HIF-1 alpha at 'Asp-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation … css change child when parent hover

大鼠低氧诱导因子1α（HIF-1α）ELISA检测试剂盒_大鼠 ...

WebFigure 1. HIF-1a staining of HeLa cells. Anti-HIF-1a antibody [EPR16897] (ab179483) was used at 1/50 dilution with goat anti-rabbit IgG (Alexa Fluor ® 488) secondary antibody. Cells were treated with 0.6 nM CoCl 2 for 6 hours. Application. Recommended product. Western blot, immunocytochemistry. Anti-HIF-1a antibody (ab179483) ELISA. WebHIF-1 is a heterodimer consisting of an alpha and beta subunit, both belonging to the basic-helix-loop-helix Per-aryl hydrocarbon receptor nuclear translocator-Sim (PAS) family of … WebWB recognizes a band in hypoxic samples at ~115 kDa. HIF-1 alpha protein (NBC1-18422) has been successfully used for antibody preadsorption/blocking assay in IHC-P with HIF-1 alpha antibody NB100-479. Flow Cytometry usage reported in scientific literature (PMID 21917971). Use in Immunoblotting reported in scientific literature (PMID 28506759). css change child element on parent hover css

ZnO Nanorods Create a Hypoxic State with Induction of HIF-1 and …

HIF-1 alpha Antibody - BSA Free (NB100-479): Novus Biologicals

Web23 de jul. de 2024 · Datasheets. HIF1a Antibody (28b) is a mouse monoclonal IgG 1 κ HIF1a antibody, cited in 241 publications, provided at 200 µg/ml. epitope mapping within … WebLane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the Mouse Anti- Human HIF-1α antibody. Show More Show Less ... (HIF-1). HIF-1 is a heterodimer whose α and β subunits are members of the PAS family of basic helix-loop-helix (bHLH) transcription factors. css change child on focus of parentWebProduct Specific Information. Description: This Mgc3 monoclonal antibody detects hypoxia-inducible factor 1 alpha (HIF-1 alpha) from human, non-human primate, bovine, mouse and porcine cells. This antibody does not cross-react with ARNT or the related HIF-2 alpha. Mgc3 has been successfully used in western blotting, immunofluorescence ... ear drops pediatric pull pinna

"WebDetection of HIF‑1 alpha in MCF‑7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line treated with CoCl 2 was stained with Mouse Anti-Human/Mouse HIF-1a PE-conjugated Monoclonal Antibody (Catalog # IC1935P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram).To facilitate intracellular staining, … " - Hif 1a antibody

Hif 1a antibody

HIF1A Monoclonal Antibody (H1alpha67) - Thermo Fisher Scientific

WebHIF1A Polyclonal Antibody, Invitrogen™-Rabbit Polyclonal Antibody Achetez HIF1A Polyclonal Antibody, Invitrogen™ sur Fishersci.fr Français ... HIF-1 is a heterodimer consisting of an alpha and beta subunit, both belonging to the basic-helix-loop-helix Per-aryl hydrocarbon receptor nuclear translocator-Sim (PAS) ... Web11 de nov. de 2015 · We identified HIF-1α binding sites across the genome, with binding sites showing enrichment for an RCGTG motif, showing conservation with the mammalian hypoxia response element. Conclusions: Transcriptome analysis of vhl mutant embryos detected activation of key hypoxia response genes seen in human cell models of …

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WebHIF-1 alpha Antibody detects endogenous levels of total HIF-1 alpha. Immunogen: A synthesized peptide derived from human HIF-1 alpha. Description: Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses ... WebThis antibody does not cross-react with ARNT or the related HIF-2 alpha. MA1-516 has been successfully used in Western blot, immunofluorescence, immunoprecipitation, immunocytochemistry, and gel shift procedures. By Western blot, this antibody detects an ∽116 kDa protein representing HIF-1 alpha after hypoxic induction in COS cells.

WebLane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the Mouse Anti- Human HIF-1α antibody. Show More Show Less ... (HIF-1). HIF-1 is a heterodimer whose α and β subunits are members of the PAS family of basic helix-loop-helix (bHLH) transcription factors. WebHypoxia-inducible factor 1 (HIF1) is a heterodimeric transcription factor that plays a critical role in the cellular response to hypoxia (1). The HIF1 complex consists of two subunits, HIF-1α and HIF-1β, which are basic helix-loop-helix proteins of …

WebProduct Description. Hypoxia-inducible factor- 1α (HIF- 1α) is a transcription factor that accumulates under low- oxygen conditions. 1, 2 Following hypoxic stimulus and cytoplasmic accumulation, HIF- 1α migrates to the nucleus where with other transcription factors, it drives the production of stress- adaptive proteins. WebRabbit polyclonal HIF-1 alpha antibody. Validated in WB and tested in Recombinant fragment. Cited in 46 publication(s). Independently reviewed in 1 review(s). Immunogen …

WebSummary. HIF1 alpha antibody recognizes HIF1 alpha (HIF1a) protein, which is a subunit of the heterodimeric transcription factor hypoxia-inducible factor-1 (HIF-1). HIF-1 is a …

WebHIF1A Antibodies Antibodies that detect HIF1A can be used in several scientific applications, including Immunohistochemistry, Western Blot, Immunocytochemistry, Flow … ear drops post tubesWebHIF-1 or hypoxia inducible factor 1 (predicted molecular weight 93kDa), is a transcription factor commonly referred to as a "master regulator of the hypoxic response" for its … css change by idWebHIF-1α Antibody 可检测 HIF-1α 总蛋白的内源水平。该抗体在生理条件不与其他家族成员发生交叉反应，并且不会检测显著水平的羟化 HIF-1α。物种反应性：人, 猴来源/纯化使 … css change color after secondsWebCytoplasmic in normoxia, nuclear translocation in response to hypoxia. Specificity. This antibody is directed against human HIF-1a hydroxyP564 and is specific for the hydroxylated form of the protein. Minimal reactivity occurs with the non-hydroxylated form of the protein. This antibody is specific for HIF-1a hydroxylated at P564. ear drops steroid and antibioticsWebMonoclonal Antibody for studying HIF1A. Cited in 220 publications. Validated for WB, ChIP, ChIP, C&R. Highly specific and rigorously validated in-house, HIF-1α (D2U3T) Rabbit Monoclonal Antibody (CST #14179) … ear drops that are ototoxicWebFigure 1: Western Blot - Anti-HIF1A Antibody (A11945) Western blot analysis of extracts of various cell lines, using Anti-HIF1A Antibody (A11945) at 1:1,000 dilution. Secondary … ear drops that are not ototoxicWeb保存条件： HIF-1alpha/HIF1A Mouse Monoclonal Antibody -20ºC保存，需避免反复冻融。Western一抗稀释液-20℃或4℃保存，一年有效，Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。 css change color animation