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Hieff clone plus multi one step cloning kit

Web20 de mai. de 2024 · The purified PCR product was then cloned into the pCAMBIA1300-UBI-RBCS vector using the Hieff Clone® Plus One Step Cloning Kit. The D3 knockout vector was constructed using the CRISPR/Cas9 method. Based on the genomic sequence of D3 gene, two specific CRISPR target sites were designed in the protein-encodable … Web9 de dez. de 2024 · Then, the upstream (before the start codon of the gene) and downstream (after the stop codon of the gene) fragment of each gene and the enzymatically digested vector were mixed together by seamless cloning with the Hieff Clone® Plus Multi One Step Cloning Kit (YEASEN, Shanghai, China), to get the plasmids pKC1139S …

Cloning Bioline Meridian Bioscience

WebUniversal one step cloning kit, a one step cloning kit, is developed by seamless cloning technology based on homologous recombinase. Compared with the traditional enzyme ligation technology, the seamless cloning kit is simple and fast to operate, and is not limited by the restriction of the enzyme cleavage site. It can splicing multiple fragments at one … Web11 de nov. de 2024 · The In-Fusion cloning based on in vitro homologous recombination was performed to generate all the constructs using an In-Fusion HD Cloning Kit (Clontech) and Hieff Clone Plus Multi One Step ... ready to love cast members season 3 https://hartmutbecker.com

Hieff Clone Plus Multi One Step Cloning Kit Cat No. 10912

Web17 de mar. de 2024 · Multi-dimensional proteomics outlines systematic cellular response to rapamycin ... Hieff Clone Plus Multi One Step Cloning Kit: Yeasen: Cat#10912ES10: PolyJet reagent: SignaGen: Cat#SL100688: PCR purification kit: QIAGEN: Cat#EN0531: SYBR Green Supermix: Thermo Fisher Scientific: Cat#34364344: WebDescription. This kit is further developed based on the principle of efficient and rapid connection of DNA fragments by topoisomerase. Compared with the traditional T4 ligase, … http://yeasen.com/assets/upload/file/20240907084815442.pdf how to take my iphone off do not disturb

Hieff CloneTM一步法同源重组快速定向克隆试剂盒 - 产品 ...

Category:Takara Bio USA, Inc. In-Fusion® HD

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Hieff clone plus multi one step cloning kit

TA Cloning Kits Thermo Fisher Scientific - US

WebHieff Clone® Plus Multi One Step Cloning Kit是一款简便、快速、高效的DNA定向克隆产品，该试剂盒可以将PCR产物定向克隆至任何载体的任何位点，可高效克隆50 bp-10 kb … Web产品描述. Hieff Clone TM Plus Multi One Step Cloning Kit 是一款简便、快速、高效的 DNA 定向克隆产品，该试剂盒可以将 PCR 产物定向克隆至任何载体的任何位点 ，可高效克隆 50 bp-10 kb 片段。 将 载体线性化，并在插入片段正、反向 PCR 引物 5’ 端引入 15-25 bp 的线性化载体末端同源序列，使得插入片段 PCR 产物 ...

Hieff clone plus multi one step cloning kit

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WebGostaríamos de lhe mostrar uma descrição aqui, mas o site que está a visitar não nos permite. Web1 de jul. de 2024 · Secondly, fragments of EGFP coding sequence (amplified with EGFP-ORF-F and EGFP-ORF-R) and Pxvasa 3′UTR (amplified with vasa3′UTR-F and vasa3′UTR-R) were used to construct two transient expression vectors vasaP2k-EGFP-SV40 and vasaP1214-EGFP-vasa3′UTR using Hieff Clone Plus Multi One Step Cloning Kit …

WebVazyme ClonExpress II One Step Cloning is a simple, fast, and high efficient cloning technology and enables directional insertion of any amplified DNA product into any linearized vector at any site. Firstly, the vector is linearized at the cloning site. A small sequence overlapped with each end of the cloning site is added onto the insert ... http://yeasen.com/assets/upload/file/20240817130924833.pdf

Webcloned into the HindIII/EcoRI sites of shuttle vector pWHU2581 using Hieff Clone® Plus Multi One Step Cloning Kit (Yeasen, China). The gene (aprI, aprJ, aprZ) knockout … WebBGC0001040: rapamycin biosynthetic gene cluster from Streptomyces rapamycinicus NRRL 5491.

Web12 de ago. de 2024 · The full-length cDNA of OsEBF1 (LOC_Os06g40360) was amplified with KOD-plus DNA polymerase (Toyobo, Tokyo, Japan) using the primers EBF1OE-attB-F and EBF1OE-attB-R, and cloned into the p1301-35SNos vector using a Hieff-Clone™ Plus Multi One Step Cloning Kit (Yeasen, Beijing, China).

WebIn-Fusion® HD EcoDry™ Cloning Kit User Manual (080318) takarabio.com Takara Bio USA, Inc. Page 3 of 14 I. Introduction In-Fusion HD EcoDry Cloning Kits are designed for fast and convenient, directional cloning of one or more fragments of DNA into any vector. The cornerstone of In-Fusion Cloning technology is our proprietary In-Fusion how to take my ged testWebItem Hieff Clone® Plus Multi One Step Cloning Kit; Company Yeasen Biotechnology Co., Ltd; Catalog Number 10912; This product is no longer available on Biocompare. … how to take my name off a joint bank accountWeb14 de fev. de 2024 · 图8：Hieff Clone TM Plus Multi One Step Cloning Kit与不同品牌的同类产品相比，显示出更高的性价比。 FAQ. Q1 ： Hieff Clone TM 克隆的原理是什 … how to take my pc back to an earlier dateWebHieff Clone® Plus One Step Cloning Kit是一款简便、快速、高效的DNA定向克隆产品，该试剂盒可以将PCR产物定向克隆至任何载体的任何位点，可高效克隆50 bp-10 kb片段。 … how to take my name off an llcWeb27 de abr. de 2024 · PCR of the coding sequence of Met or Gce from the pClone007-Met or pClone007-Gce vector was performed, and then we used a Hieff Clone Plus Multi One Step Cloning Kit (10912ES10, Yeasen) for assembly. Finally, we obtained the pUAST-Met-4 × FLAG-Avi-P2A-BirA ( UAS-Met-BirA ) or pUAST-Gce-3 × FLAG-Avi-P2A-BirA ( UAS … how to take my number off call listsWeb17 de set. de 2024 · On one side, DRAM1 interacted ... BioID2 plasmid, respectively. 3FLAG-DRAM1 and GCaMP6 or DRAM1 and BioID2-FLAG were homologously recombined using the Hieff Clone® Plus Multi One Step Cloning Kit ... how to take my pc out of safe modeWebToo lazy to do cloning work? Empower your laziness with our Pro Ligation-free Cloning Kit! This bestselling kit offers:1) One-step cloning - no reliance on r... ready to love clifton and joy