WebGrowth Strain (s) Stbl3 Copy number Low Copy Gene/Insert Gene/Insert name miniTurbo-V5-MAST3 Alt name MAST3 Species H. sapiens (human) Entrez Gene MAST3 Promoter Ubiquitin Tag / Fusion Protein miniTurbo-V5 (N terminal on insert) Cloning Information Cloning method Gateway Cloning 5′ sequencing primer TGAAGCTCCGGTTTTGAACT WebPlasmid lentiCas9-EGFP from Dr. Feng Zhang's lab contains the inserts Cas9 and EGFP and is published in Cell. 2015 Mar 12;160(6):1246-60. doi: 10.1016/j.cell.2015.02.038. ... Growth Strain(s) Stbl3 Growth instructions. Use SapI digest to check for unwanted recombination of lentiviral plasmid. ...
pLenti-CMV-VTCN1-GFP/Puro-质粒与载体-产品中心-PPL质粒与蛋 …
WebPlasmid pLenti CMV GFP Neo (657-2) from Dr. Eric Campeau's lab contains the insert GFP and is published in PLoS One. 2009 Aug 6;4(8):e6529. This plasmid is available through Addgene. ... Growth Strain(s) Stbl3 Growth instructions. Stbl3, 37oC Copy … WebGrowth Strain(s) Stbl3 Copy number. High Copy Gene/Insert Gene/Insert name. scFv-GCN4 Insert Size (bp) 828 Resource Information. Articles Citing this Plasmid. 41 References; Terms and Licenses. Academic/Nonprofit Terms. UBMTA; Ancillary Agreement for Plasmids Containing FP Materials ... head scarves crossword clue
Addgene: pL-CRISPR.EFS.GFP
WebGrowth Strain (s) Stbl3 Copy number High Copy Gene/Insert Gene/Insert name Cre Species Synthetic Insert Size (bp) 1053 Promoter Ef1a Cloning Information Cloning method Restriction Enzyme 5′ cloning site BamHI (not destroyed) 3′ cloning site EcoRI (not destroyed) 5′ sequencing primer CACCCACACAAAGGAAAAGGGCC http://www.geneppl.com/productinfo.php?id=758793 WebGrowth Temperature 37°C Growth Strain (s) Stbl3 Growth instructions Stbl3 or SC110 (See below**) Copy number Unknown Gene/Insert Gene/Insert name None Tag / Fusion Protein EGFP (N terminal on backbone) Cloning Information Cloning method Restriction Enzyme 5′ cloning site XbaI/NheI (not destroyed) 3′ cloning site BamHI/BcII (not destroyed) goldthorpe academy