Cell lines for honey bee virus research
WebAug 12, 2008 · Skilled in multidisciplinary research (molecular biology, microbiology, chemistry and cell biology), Flenniken is focusing on the biology of honeybee viruses, specifically the role of RNA interference (RNAi) in the … WebIn addition, further use and development of immortalized honey bee lines (i.e., AmE-711) [134 ••], long-term cell cultures [135], and primary cell cultures [136,137], are required to further the field of honey bee virology. Future use of immortalized cell lines and infectious honey bee virus clones will serve to normalize future studies and ...
Cell lines for honey bee virus research
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WebDec 24, 2009 · A media for the production of cell cultures from hymenopteran species such as honey bee, Apis mellifera L. (Hymenoptera: Apidae) was developed. Multiple bee cell cultures were produced when using bee larvae and pupae as starting material and modified Hert–Hunter 70 media. Cell culture systems for bees solves an impasse that has … WebOct 20, 2024 · The cell line AmE-711 is persistently infected with the honey bee positive-sense, single-stranded RNA virus, Deformed wing virus (Carrillo-Tripp et al., 2016), a common pathogen of honey bee colonies. …
WebSep 21, 2024 · An AmE-711 honey bee cell undergoing division. a A parent honey bee cell in late metaphase/early anaphase of the cell cycle where the chromosomes have duplicated to form pairs of sister chromatids. The pairs of chromatids are lined up along the equator of the cell, marked with a blue arrowhead.The pairs of chromatids will eventually split, and … WebJan 15, 2024 · The establishment of virus-free, honey bee cell lines will facilitate a number of avenues of research including (1) screening for antiviral compounds, (2) screening for …
WebExplore Scholarly Publications and Datasets in the NSF-PAR. Search For Terms: × WebAug 18, 2016 · Recently, an immortalized honey bee cell line (AmE-711) was utilized for the first time to investigate host—virus and virus—virus interactions in mixed infections . …
WebFeb 18, 2024 · Sacbrood virus (SBV) of honey bees is a picornavirus in the genus Iflavirus. Given its relatively small and simple genome structure, single positive-strand RNA with only one ORF, cloning the full genomic sequence is not difficult. However, adding nonsynonymous mutations to the bee iflavirus clone is difficult because of the lack of …
WebJan 17, 2024 · A continuously replicating cell line derived from the honey bee would provide a valuable tool for study of molecular mechanisms of virus – host interaction, for screening of antiviral agents for potential use within the hive, and for assessment of the risk of current and candidate insecticides to the honey bee. bwc powder coatingWebOct 10, 2024 · However, cell lines capable of sustained replication of honey bee viruses are very scarce. Guo et al. [ 4 ] provide a comprehensive review on the status of A. mellifera cell lines, as well as other cell lines that might be helpful, including a description of ways to develop new cell lines. bwc propertyWebJul 23, 2013 · The honey bee cell line reported below is an original description of a line that was developed by the authors at the University of Minnesota from honey bee embryos. ... Genersch E, Gisder S, Hedtke K, Hunter WB, Möckel N et al. 2013). Standard Methods for cell cultures in Apis mellifera research. J Apic Res 52:1-8. ... Honey bee viruses. … bwc printsWebThus far, honey bee virus studies have provided valuable information on the response of hosts to viruses at the population and physiological levels but immortalized driven honey bee cell lines will provide a stable supply of material to a nascent field of honey bee cellular virology (de Miranda et al., 2013; Genersch et al., 2013; Martin and ... cf 126b passwordWeb49 minutes ago · Background: Microsporidia from the Nosema (Vairimorpha) genus are pathogenic fungi that complete their life cycle in the honeybee intestine. Therefore, the aim of this study was to determine the impact of the course of infection on the viability of honeybee intestine cells. Methods and Results: Intestines isolated from healthy and N. … bwc re250t6-1ncwwWebSep 26, 2024 · With incoming honey bee cell lines , efforts in finding alternative host cells, and clarifications of viral polyprotein cleavages, more applications of virus clones in honey bee research are expected, including anti-virus responses, virus/host interaction, and ecology. Such studies may elucidate the role of virus diseases in population declines ... bwc provider searchWebApr 14, 2024 · A ctivists often cite the alleged health risks of genetically modified foods. A classic example of this—”10 Scientific Studies Proving GMOs Can Be Harmful To Human Health,” posted on ... bwc printworks